Recombinant Human TRAIL R4 / CD264 / TNFRSF10D Protein (His tag)

Product information

Size

100µg

Catalog number

PKSH031621-100µg

Price

445 EUR

More information

Synonym

CD264;DCR2;TRAIL-R4;TRAILR4;TRUNDD

Activity

Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10D at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.625-40 ng/ml.2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 0.05-0.5 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.

Sequence

Met 1-His 211

Fusion tag

C-His

Accession

NP_003831.2

Expressed Host

HEK293 Cells

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Purity

>90 % as determined by SDS-PAGE

Endotoxin

<1.0 EU per µg as determined by the LAL method

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Mol Mass

18.4 kDa

AP Mol Mass

30-40 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Background

Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-κB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.

Conjugation

histidine

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants