SQSTM1 Antibody, FITC Conjugated

Product information

Size

0.1ml

Catalog number

bs-2951R-FITC

Price

333 EUR

More information

Long name

SQSTM1 Polyclonal Antibody, FITC Conjugated

Category

Conjugated Primary Antibodies

Conjugation

FITC

Host Organism

Rabbit (Oryctolagus cuniculus)

Target Antigen

SQSTM1

Specificity

This is a highly specific antibody against SQSTM1.

Modification

Unmodified

Modification site

None

Clonality

Polyclonal

Clone

Polyclonal antibody

Concentration

1ug per 1ul

Immunogen_range

80-130/440

Subcellular location

Cytoplasm, Nucleus

Source

KLH conjugated synthetic peptide derived from human SQSTM1/Ubiquitin-binding protein p62

Gene ID number

8878

Swiss Prot

Q13501

Tested applications

IF(IHC-P)

Recommended dilutions

IF(IHC-P)(1:50-200)

Crossreactivity

Human, Mouse, Rat

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Antigen background

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Purification method

This antibody was purified via Protein A.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Excitation Emission

494nm/518nm

Synonyms

p60; p62; A170; OSIL; PDB3; ZIP3; p62B; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.

Additional conjugation

Anti-FITC Antibody

French translation

anticorps